Journal: IBRO neuroscience reports

Article Title: IL-33 relieves nerve injury by mediating microglial polarization in neuromyelitis optica spectrum disorders via the IL-33/ST2 pathway.

doi: 10.1016/j.ibneur.2024.07.008

Figure Lengend Snippet: Fig. 5. Microglial cells were activated and transformed to the M2 phenotype after treatment with IL-33. (a) Western blot images and the quantification of CD206 and CD40 levels after treatment with IL-33 or NMOD serum. (b) BV2 cells were labeled with Iba-1 and CD68 for immunofluorescence. (c) Expression levels of PSD95 were observed by immunofluorescence after treatment with IL-33 or NMOSD serum. One-way analysis of variance was used for statistical analyses. *p<0.05, **p<0.01, ***p<0.001.

Article Snippet: For protein immunoreactivity in cells, the primary cortical neurons or BV2 cells were fixed with 4 % paraformaldehyde for 30 min at RT and treated with 0.5 % Triton for 10 min. After being washed with PBS, the cells were blocked with 5 % bovine serum albumin (BSA) for 1 h and then incubated with a rabbit antiPSD95 antibody (1:100 dilution, PA2295, Boster), anti-CD68 antibody (1:200 dilution, 28058–1-AP, Proteintech), anti-IBA1 antibody (1:100 dilution, CY7217, Abways), or anti-ST2 antibody (1:200 dilution, PRS3363, Sigma) at 4 ◦C overnight.

Techniques: Transformation Assay, Western Blot, Labeling, Immunofluorescence, Expressing

Journal: PNAS nexus

Article Title: Commensal microbiome dysbiosis elicits interleukin-8 signaling to drive fibrotic skin disease.

doi: 10.1093/pnasnexus/pgae273

Figure Lengend Snippet: Fig. 5. Fibroblasts are the feasible source of IL-8 in keloids. A) Percentage of patients in tested IL-8 chemokine subgroup in circulation. Reference values (RVs) are as follows: IL-8 = 0–20.60 pg/mL. The number of patients (n = 66) is indicated. B) Representative fluorescence image of samples from five patients showing the simultaneous detection of α-SMA, CD68, CD45, IL-8, and DAPI in human keloid. Scale bars, 100 μm. Magnification is 40×. C) Statistic analysis of indicated cell percent based on mIHC staining data from B). Mean ± SEM. nsP > 0.05; *P < 0.05. Normality and log-normality tests followed by two-tailed unpaired Student’s t test. D) Cell proximity analysis of indicated cells in the given distance based on mIHC staining data from B). Quantitative immunofluorescence was processed by HALO software. E) Dermal fibroblasts isolated from keloid tissues were treated with variable dosages of HKST for 24 h. IL-8 concentration in the supernatant was measured by ELISAs, n = 3 different keloid samples as a source of cells per subgroup. Normality and log-normality tests followed by one-way ANOVA were performed for the statistical analysis. Mean ± SEM. *P < 0.05. F) Keloid fibroblasts were treated with variable dosages of HKSA for 24 h. IL-8 concentration in the supernatant was measured by ELISAs, n = 3 per subgroup. Normality and log-normality tests followed by one-way ANOVA were performed for the statistical analysis. Mean ± SEM. nsp > 0.05; *P < 0.05; **P < 0.01.

Article Snippet: Formalin-fixed and paraffin-embedded (FFPE) sections were evaluated by mIHC analysis following previously reported protocol (73) using antibodies for α-SMA (ab124964, Abcam, 1:500), IL-8 (MAB208, R&D Systems, 1:100), CD45 (2403794, Invitrogen, 1:200), and CD68 (76437, Cell Signaling Technology, 1:400).

Techniques: Fluorescence, Staining, Two Tailed Test, Immunofluorescence, Software, Isolation, Concentration Assay

Journal: EMBO Molecular Medicine

Article Title: Domain-substituted IGF2 tag modulates targeting of lentiviral gene therapy for Hunter syndrome

doi: 10.1038/s44321-025-00314-3

Figure Lengend Snippet: ( A ) LAMP1 staining in heart valves and aortic wall. n = 3. Scale bar = 100 µm. ( B – E ) Microarchitecture analysis of the medial cuneiform . ( F ) 3D rendering of reconstructed μCT scans of the medial cuneiform . ( G ) Explanatory drawings of μCT parameters. The volume/area measured by the μCT parameters is highlighted in blue. ( H ) VCN per genome in bone marrow of mice used for histological analysis of peripheral tissues. ( I ) Dermatan sulfate levels in total brain homogenates measured by mass-spectrometry. ( J , K ) VCN per genome in bone marrow of mice used for immunostaining of LAMP1 and GFAP ( J ), or CD68 ( K ) in brain. Data information: data are presented as means ± SD and were analyzed by one-way ANOVA with Bonferroni’s correction. In ( B – E ) n = 6; in ( H , J , K ) n = 3; in ( I ) n = 6 ( IDSco, IDS.IGF2co, GFP, Ids y/− , WT) or n = 7 ( IDS.IGF2del_co, IDS.SWAP-ApoEco, IDS.SWAP- RAP12x2co). Tb: trabecular bone; T: trabeculae; Cb: cortical bone. Data are presented as means ± SD and were analyzed by one-way ANOVA with Bonferroni’s correction.

Article Snippet: Rat anti-CD68 primary antibody , Bio-Rad , MCA1957T.

Techniques: Staining, Mass Spectrometry, Immunostaining

Journal: EMBO Molecular Medicine

Article Title: Domain-substituted IGF2 tag modulates targeting of lentiviral gene therapy for Hunter syndrome

doi: 10.1038/s44321-025-00314-3

Figure Lengend Snippet: Example of sagittal brain sections from GFP-treated mice stained for LAMP1 ( left panel ), GFAP ( central panel ) or CD68 ( right panel ). n = 3. Scale bar = 1 mm.

Article Snippet: Rat anti-CD68 primary antibody , Bio-Rad , MCA1957T.

Techniques: Staining

Journal: EMBO Molecular Medicine

Article Title: Domain-substituted IGF2 tag modulates targeting of lentiviral gene therapy for Hunter syndrome

doi: 10.1038/s44321-025-00314-3

Figure Lengend Snippet: Representative images of CD68 staining of sagittal sections of cortex, hippocampus, thalamus, midbrain, brainstem and cerebellum of gene therapy-treated Ids y/− mice and controls. Scale bars = 100 µm. Quantification of CD68-positive cells is shown. VCN in bone marrow of the mice used for histology is shown in Fig. . An example of CD68 staining on GFP -treated Ids y/− mice is shown in Fig. . All significant comparisons in showed P values < 0.0001, except for: Cortex , IDSco vs. Ids y/ − P = 0.0210, IDS.IGF2co vs. Ids y/− P = 0.0003, IDS.IGF2del_co vs. Ids y/− P = 0.0201. Thalamus, IDSco vs. WT P = 0.0047, IDS.IGF2del_co vs. WT P = 0.0123, IDS.SWAP-ApoEco vs. WT p = 0.0038. Midbrain, IDS.IGF2co vs. Ids y/− P = 0.0093, IDS.SWAP-ApoEco vs. Ids y/− P = 0.0141, IDS.SWAP-RAP12x2co vs. Ids y/− P = 0.0081. Cerebellum , IDSco vs. Ids y/− P = 0.0016, IDS.IGF2co vs. Ids y/− P = 0.0003, IDS.IGF2del_co vs. Ids y/− P = 0.0010, IDS.SWAP-ApoEco vs. Ids y/− P = 0.0031. Data information: data are presented as means ± SD and were analyzed by one-way ANOVA with Bonferroni’s correction. Asterisks (*) represent significance versus WT; hashes (#) represent significance versus Ids y/− . n = 3. * P ≤ 0.05; ** P ≤ 0.01; **** P ≤ 0.0001. # P ≤ 0.05; ## P ≤ 0.01; #### P ≤ 0.0001. Significant results are indicated by brackets. .

Article Snippet: Rat anti-CD68 primary antibody , Bio-Rad , MCA1957T.

Techniques: Staining

Journal: EMBO Molecular Medicine

Article Title: Domain-substituted IGF2 tag modulates targeting of lentiviral gene therapy for Hunter syndrome

doi: 10.1038/s44321-025-00314-3

Figure Lengend Snippet: Representative images of GFAP (green) staining of sagittal sections of cortex, hippocampus, thalamus, midbrain and brainstem of gene therapy-treated Ids y/− mice and controls. Scale bar = 100 µm. Nuclei are stained in red. Dashed lines outline the Cornu Ammonis (CA) fields 2 and 3. Quantification of GFAP fluorescence area is shown. VCN in bone marrow of the mice used for histology is shown in Fig. . An example of CD68 staining on GFP -treated Ids y/− mice is shown in Fig. . All significant comparisons in showed P values < 0.0001, except for: Cortex , IDSco vs. WT = 0.0239, IDS.IGF2del_co vs. WT P = 0.0434, IDSco vs. Ids y/− = 0.0023, IDS.IGF2del_co vs. Ids y/ − P = 0.0012. Hippocampus , IDSco vs. WT P = 0.0140, Ids y/− vs. WT P = 0.0010, IDS.SWAP-ApoEco vs. Ids y/− P = 0.0007, IDSco vs. IDS.SWAP-ApoEco P = 0.0106. Brainstem, IDSco vs. WT P = 0.0002, Ids y/− vs. WT P = 0.0087, IDSco vs. IDS.IGF2co P = 0.0033, IDSco vs. IDS.SWAP-ApoEco P = 0.0037, IDSco vs. IDS.SWAP-RAP12x2co P = 0.0110. Data information: data are presented as means ± SD and were analyzed by one-way ANOVA with Bonferroni’s correction. Asterisks (*) represent significance versus WT; hashes (#) represent significance versus Ids y/− . n = 3 . * P ≤ 0.05; ** P ≤ 0.01; *** P ≤ 0.001; **** P ≤ 0.0001. # P ≤ 0.05; ## P ≤ 0.01; ### P ≤ 0.001; #### P ≤ 0.0001. Significant results are indicated by brackets. .

Article Snippet: Rat anti-CD68 primary antibody , Bio-Rad , MCA1957T.

Techniques: Staining, Fluorescence

Journal: PLoS ONE

Article Title: Cholesterol Diet Withdrawal Leads to an Initial Plaque Instability and Subsequent Regression of Accelerated Iliac Artery Atherosclerosis in Rabbits

doi: 10.1371/journal.pone.0077037

Figure Lengend Snippet: Panel (A) Representative images of hematoxylin and eosin stained sections of all groups (Scale bar = 500 µm). Panel (B) Representative images of Oil Red O stained cryostat sections of all groups (Scale bar = 50 µm). Panel (C) Immunohistochemical staining showing CD68 positive cells in respective groups. (Scale bar = 50 µm) (D) Quantitative analysis of percentage lipid area (lumen area included) in respective groups (E) Quantitative analysis of CD68 positive labelling in respective groups. **p<0.01 and ***p<0.001 vs normal; ## p<0.01 and ### p<0.001 vs Baseline. (* indicates lumen, arrow heads indicate positive staining)

Article Snippet: Paraffin-embedded consecutive sections were stained with the following antibodies: Mouse monoclonal anti rabbit CD68 (RAM11, Dako, USA), alpha smooth muscle actin (1A4, Sigma, USA) and MMP-9 (56-2A4, Calbiochem, USA).

Techniques: Staining, Immunohistochemical staining